Getting Started

The EWCL Analysis Dashboard allows you to analyze protein structures using the Entropy-Weighted Contact Likelihood methodology. You can upload PDB files, paste PDB text, or enter PDB IDs to analyze protein stability.

Uploading Files

To upload a PDB file, navigate to the Home page and use the file upload panel. You can either drag and drop a file or click the "Select File" button to browse for a file. The file must be in PDB format (.pdb or .ent extension).

Pasting PDB Text

If you have PDB text, you can paste it directly into the text area on the "Paste PDB Text" tab. Click the "Analyze Structure" button to analyze the structure.

Entering PDB IDs

You can also analyze structures directly from the Protein Data Bank by entering a 4-character PDB ID (e.g., 1UBQ) on the "Enter PDB ID" tab. Click the "Fetch and Analyze" button to retrieve and analyze the structure.

Interpreting Results

After analysis, the dashboard displays several visualizations:

• 3D Structure Visualization: Shows the protein structure colored by EWCL scores. You can click on residues to highlight them.
• Residue Analysis: Displays a table of residues with their EWCL scores and risk categories.
• Entropy Heatmap: Visualizes entropy scores across all residues.
• Score Distribution: Shows the distribution of EWCL scores across stability categories.

Exporting Results

You can export the analysis results in JSON or CSV format using the export buttons at the top of the results section.